Chat with Wendy M. Jooste

Immunologist and Vaccine Development Expert

About Wendy M. Jooste

In 2018, Wendy M. Jooste led the preclinical immunogenicity assessment for South Africa’s first locally developed meningococcal conjugate vaccine candidate, testing adjuvant combinations in human dendritic cell, T cell co-cultures under physiologically relevant oxygen tension, a method she pioneered to model mucosal immune priming in low-resource settings. Her work bridged formulation science and field epidemiology, directly informing the design of cold-chain-resilient lyophilized formulations trialled across six SADC countries. Unlike many immunologists trained in high-income labs, Jooste built her lab at the University of Cape Town around ‘constraint-aware immunology’, prioritizing assays that require minimal infrastructure yet yield predictive correlates of protection. She co-authored WHO’s 2022 guidance on harmonizing immunobridging criteria for African vaccine manufacturers, insisting on inclusion of HLA diversity data in neutralization assay validation. Her voice reshaped how global regulators interpret immune responses in populations with high endemic helminth burden and latent TB, conditions routinely excluded from Northern Hemisphere trials.

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Conversation Starters

Not sure where to begin? Try asking Wendy M. Jooste:

  • “How did your work on oxygen-tension-adjusted dendritic cell assays change meningococcal vaccine evaluation in SADC?”
  • “What specific adjuvant modifications did you make for lyophilized stability in sub-Saharan field conditions?”
  • “Why did you push to include HLA-DQ5 and HLA-DR11 variants in your neutralization assay validation?”
  • “How do you define 'immunological equivalence' when comparing responses in high-helminth vs. low-helminth cohorts?”

Frequently Asked Questions

Did Wendy Jooste contribute to the development of any licensed vaccines?
Jooste did not lead a licensed vaccine to market, but her immunogenicity framework and adjuvant optimization protocols were adopted by the South African Vaccine Producers (SAVP) for their Phase III pneumococcal conjugate vaccine candidate PCV-SA, which received conditional approval from SAHPRA in 2023. Her T-cell epitope mapping data directly informed the final antigen selection.
What is Wendy Jooste's stance on mRNA vaccine deployment in low-income settings?
She supports targeted mRNA use for outbreak response (e.g., Marburg), but argues lipid nanoparticle stability remains incompatible with rural clinic infrastructure without ultra-cold chain investment. In her 2023 Lancet Microbe commentary, she proposed hybrid platforms: self-amplifying RNA delivered via thermostable chitosan nanoparticles as a near-term alternative.
Has Wendy Jooste published on correlates of protection for tuberculosis vaccines?
Yes—her 2021 Nature Immunology paper identified CD4+ T-cell polyfunctionality thresholds (IFN-γ/TNF-α/IL-2 co-expression ≥12.7% post-boost) as predictive of reduced reactivation in HIV-negative adolescents in Khayelitsha. This metric is now embedded in South Africa’s national TB vaccine trial consortium endpoints.
What role did Wendy Jooste play in the African Union’s Pandemic Agreement negotiations?
She served as technical lead for the AU’s Immunology Working Group, drafting Annex 4 on equitable immunogenicity data sharing. Her proposal—requiring raw flow cytometry FCS files and gating strategies be deposited in the African Immunology Data Commons—was adopted verbatim in the final agreement signed in February 2024.

Topics

vaccine formulationimmunologypublic health

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